Cloning with Liquid Media

In the same way to can clone a mushroom onto agar you can culture directly into liquid culture from a fresh fruitbody, In short you are making a biopsy of a mushroom using a syringe needle and injecting that sample through the airport lid.

Materials:

  • 1 airport jar with steriles liquid meida
  • 1 airport jar with fresh water
  • 1 syringe with needle (16-18 guage)
  • Tin foil
  • Alcohol spray (ISO Propynol diluted to 70%)
  • 1 fresh mushroom

 

Method:

  1. Sterilise the jars, syringe and needle (if re-using glass syringe and steel needle). Allow to cool.
  2. Aerate Liquid media jar (see Liquid Culture Growth and Development)
  3. Spray both silcone injection sites with alcohol.
  4. Using syringe draw up 5ml of water.
  5. Making sure you have cleaned the mushroom, slice it in half and using the needle stab the fresh inner flesh. Check that there is tissue in the needle shaft. Repeat until you can see tiisue in the needle.
  6. Insert the needle through the airport lid and inject in the jar.
  7. Label and date the jar to be stored in your incubation space

 

 

13 Comments

  1. Hello there! Do you know if they make any plugins to assist with Search Engine Optimization? I'm trying to get my blog to rank for some targeted keywords but I'm not seeing very good success. If you know of any please share. Thank you! You can read similar art here: Sklep
  2. Hey! Do you know if they make any plugins to assist with SEO? I'm trying to get my blog to rank for some targeted keywords but I'm not seeing very good success. If you know of any please share. Kudos! You can read similar art here: Ecommerce
  3. Hi there! Do you know if they make any plugins to help with Search Engine Optimization? I'm trying to get my website to rank for some targeted keywords but I'm not seeing very good gains. If you know of any please share. Appreciate it! You can read similar text here: Backlinks List
  4. Wow, awesome blog format! How lengthy have you ever been running a blog for? you made running a blog glance easy. The total look of your web site is fantastic, as well as the content! You can see similar here e-commerce

Leave a Reply

Your email address will not be published. Required fields are marked *

  • No products in the cart.